Postbiotics could offer a novel therapeutic approach and may be a safer alternative to probiotics. There is growing evidence revealing the diverse clinical benefits of postbiotics in many prevalent conditions. To review the evidence for the clinical benefits of postbiotics in highly prevalent conditions and consider future potential areas of benefit. Postbiotics contain inactivated microbial cells or cell components, thus are more stable and exert similar health benefits to probiotics. Interest in postbiotics is therefore increasing. However, their use in vulnerable people is linked to possible safety concerns and maintaining their viability is difficult. Probiotics have the potential to manage these. In addition, the effective antibacterial component of YH68-CFCS is lactic acid (LA).Īn optimally operating microbiome supports protective, metabolic, and immune functions, but disruptions produce metabolites and toxins which can be involved in many conditions. difficile in the effective time interval range (0-12 h) but did not affect the expression level of C. The results showed that YH68-CFCS in combination with VAN or MTR significantly inhibited the growth, biofilm production, and toxin production of C. Meanwhile, the types and contents of organic acids in YH68-CFCS were investigated by LC-MS/MS.

difficile virulence genes tcdA and tcdB were determined by real-time qPCR method. difficile was determined by enzyme immunoassay, and the relative expressions of C. difficile under different co-administration time interval treatments were determined by optical density and crystalline violet staining, respectively.

In this study, the cell-free culture supernatant (CFCS) of Bifidobacterium breve YH68 was used in combination with vancomycin (VAN) and metronidazole (MTR) to treat C. Co-administration of probiotics and antibiotics has been used to prevent or treat primary Clostridioides difficile (pCDI), and the closer the interval between the combination, the more effective it is, but the reason behind this is unknown.

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